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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1994-1998 - 1ère partie : rapport année 1994 ArchiMer
Boudry, Pierre; Naciri, Yamama; Launey, Sophie; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous Ies membres du Réseau Génétique Mollusques (REGEMO) a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses. Le contrat de plan Etat-Région a été signé en...
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Saccostrea; Génétique population.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00044/15517/12904.pdf
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Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Rapport année 1995 ArchiMer
Boudry, Pierre; Naciri, Yamama; Heral, Maurice; Collet, Bertrand; Bougrier, Serge; Geffard, Olivier; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le programme de recherche présenté ci-dessous est le fruit d'une réflexion conjointe entre généticiens et physiologistes afin de mettre sur pied un ensemble d'expériences permettant d'étudier les bases génétiques de la variabilité des caractères impliqués dans la croissance chez l'huître creuse, Crassostrea gigas.
Tipo: Text Palavras-chave: Génétique; Sélection génétique; Génétique des populations; Croissance; Crassostrea gigas.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15518/12905.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridations et conservatoire de souches. Contrat de Plan Etat Région Poitou- Charentes 1994-1998 - Convention 95/RPC-R-57 "Génétique" - Rapport année 1995 ArchiMer
Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses.
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf
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Contrôle de la gamétogénèse des huîtres creuses et plates. Relations "reproduction" et "génétique" ArchiMer
Gerard, Andre; Naciri-graven, Yamama; Boudry, Pierre; Launey, Sophie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal.
At the end of the 1980s, after two decades of aquaculture research in molluscs, Ifremer initiated its first genetic studies. These new studies quickly revealed the limits of knowledge in aquaculture, particularly in terms of controlling oyster reproduction. This presentation attempts to summarize the available information on genetics in aquaculture. In particular, breeding practices in the 1980s involved populations of broodstock, while genetics requires modern techniques where individuals, not just populations, can be identified. Controlling reproduction at the individual level in oysters raises new questions that existing protocols cannot address, especially in terms of controlling sex determination, individual maturity, spawning synchrony, chemical...
Tipo: Text Palavras-chave: Gamètes; Fécondation; Induction ponte; Reproducteur; Conditionnement; Gamétogénèse; Génétique; Reproduction; Crassostrea gigas; Huîtres.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/acte-3210.pdf
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Genetical improvement of marine fish and shellfish : a french perspective ArchiMer
Boudry, Pierre; Chatain, Beatrice; Naciri-graven, Yamama; Lemaire, Christophe; Gerard, Andre.
In France, marine fish and shellfish farming is based mainly on the breeding of wild species, whose natural populations are traditionally exploited. Research programs are conducted by IFREMER, in co-operation with INRA and Universities. French research programs for the genetical improvement of marine fish and shellfish concern primarily seabass (Dicentrarchus labrax) and oysters (Ostrea edulis and Crassostrea gigas). The following programs are developed: •The knowledge of wild and farmed populations. The most recent prodjects involve the highly variable microsatellite markers. Seven markers are presently available for seabass and the work is now initiated on turbot (Scophthalmus maximus). In molluscs, microsatellite markers have been developed for the...
Tipo: Text Palavras-chave: Selection; Triploid; Shellfish; Fish; Genetic.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/acte-3206.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Rapport année 1996 ArchiMer
Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée).
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - First Progress Report 1st January-31st December 1996 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkinns, N. P.; Zouros, Eleftherios; Bonhomme, Francois.
The EEC project "Genephys 1996-2000", initiated by the IFREMER laboratory GAP (Génetique, Aquaculture et Pathologie, La Tremblade and CREMA (Centre de Recherche en Ecologie Marine et Aquaculture, l'Houmeau), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are: 1- To express the maximum variability of growth, both at the larval stage and at the adult stage, in an oyster population originating from parents of various origins. 2- To check the persistence over the time, of growth performance and related physiological functions. 3-...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00050/16149/13632.pdf
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Etudes des bases génétiques et de la varibailité des caractères physiologiques impliqués dans la croissance chez l'huître creuse Crassostrea gigas ArchiMer
Collet, Bertrand; Boudry, Pierre; Bougrier, Serge; Heurtebise, Serge; Phelipot, Pascal; Ledu, Christophe; Morand, Berenice; Heral, Maurice; Gerard, Andre.
A European programme (GENEPHYS) was launched in 1996 to study the genetic basis and variability of physiological characters involved in growth in Crassostrea gigas. This research programme was a prerequisite for any genetic selection programme on physiological functions. Research groups from Ireland, England, Greece and France are involved in this project and participate in genetic analyses (allozymes and microsatellites) and physiological analyses (ecophysiology, enzymology, stress proteins). Ifremer at La Tremblade was responsible for producing the biological material while Ifremer-CREMA (human-influenced marine ecosystems research centre) was responsible for carrying out non-destructive physiological analyses.
Tipo: Text Palavras-chave: Sélection génétique; Croissance; Crassostrea gias; Génétique.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3243.pdf
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Historique, expériences en cours et perspectives du programme de sélection de l'huître plate Ostrea edulis pour la résistance à la bonamiose ArchiMer
Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors.
Tipo: Text Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Second Progress Report 1st January-31st December 1997 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkins, N. P.; Zouros, Eleftherios; Bonhomme, Francois; Buestel, Dominique; Samain, Jean-francois.
The UE project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-L'Houmeau (Centre de Recherche en Ecologie marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00050/16150/13633.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huîtres creuses Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 97 RPC-R-54 "Génétique" - Rapport année 1997 ArchiMer
Boudry, Pierre; Lapegue, Sylvie; Goyard, Emmanuel; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1997 étaient les suivants : • Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. • Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant limitée).
Tipo: Text Palavras-chave: Génétique; Génétique des Populations; Acclimatation; Hybridation; Marqueurs génétiques; Sélection génétique; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00044/15519/12906.pdf
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Genetic improvement and selection in shellfish : a review based on oyster research and production ArchiMer
Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfill market demand. Up to now, the most effective answer to disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability of suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability of the shellfish industry. The different approaches to genetic improvement include...
Tipo: Text Palavras-chave: Selective breeding; Hybridization; Polyplidy; Introduction; Oysters; Selection; Hybridation; Polyploidie; Introduction; Huitres.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3499.pdf
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Ressources génétiques chez les huîtres creuses : conservatoire de souche et marqueurs moléculaires ArchiMer
Boudry, Pierre; Heurtebise, Serge; Collet, Bertrand; Cornette, Florence; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The cupped oyster strain bank was created at Ifremer at La Tremblade in 1992. It was funded by the Poitou-Charentes regional council. Its goals were: 1. to study the intra- and interspecific genetic differentiation within the genus Crassostrea. 2. to study acclimatization potential of foreign oysters in French waters in order to identify species that could replace Crassostrea gigas in case of a major disease outbreak in C. gigas or in case of a need or desire to diversify shellfish production. 3. to study the possibility of interspecific hybridisation in the genus Crassostrea and hybrid performance.
Tipo: Text Palavras-chave: Saccostrea commercialis; Crassostrea rhisophorae; Crassostrea gasar; Crassostrea sikamea; Crassostrea virginica; Crassostrea rivularis; Crassotea angulata; Crassostrea gigas; Génétique.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3242.pdf
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The use of microsatellite markers for parentage analysis in the Pacific cupped oyster, Crassostrea gigas (Thunberg) ArchiMer
Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre.
Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic...
Tipo: Text Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf
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Approche comparative de la variabilité et de la structuration génétique chez deux espèces d'huîtres cultivées (Crassostrea gigas et Ostrea edulis) présentant des stratégies reproductives et des histoires contrastées ArchiMer
Boudry, Pierre; Launey, Sophie; Huvet, Arnaud.
The two common oyster species cultivated in Europe are the flat oyster Ostrea edulis and the cupped oyster Crassostrea gigas. These species have two really different reproduction strategies (oviparity versus larviparity) and histories (natural distribution versus introductions linked to oyster culture). We used microsatellite markers to compare the observed variabilities Europe wide. O. edulis presents a lower rate of alleles per locus than C. gigas. The observed structuration levels (global Fst and by pairs values) are similar for both species, but the spatial organisation of the diversity is different. In O. edulis, appears a differentiation based on the distance (concordance of the results with those obtained with the allozymes) whereas in C. gigas,...
Tipo: Text Palavras-chave: Différenciation génétique; Variabilité; Génétique; Crassostrea gigas; Ostrea edulis; Huîtres.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-3455.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Third Progress Report 1st January-31st December 1998 - Contract N°FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Buestel, Dominique; Samain, Jean-francois; Hawkins, A. J. S.; Wilins, N.; Zouros, Eleftherios; Bonhomme, Francois; Thiriot Quievreux, Catherine.
The EU project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-l'Houmeau (Centre de Recherche en Ecologie Marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main bjectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Growth; Absorption efficiency; Oysters; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00050/16151/13634.pdf
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Acclimatation de nouvelles espèces d'huîtres creuse du genre Crassostrea: hybridation et conservatoire de souches - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Synthèse finale ArchiMer
Boudry, Pierre; Heurtebise, Serge; Huvet, Arnaud; Chollet, Bruno; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Actions menées de 1994 à 1998 au sein du conservatoire de souches : 1. Résultats des importations, acclimatation et hybridation. a) Rappel des conditions d'introduction de nouvelles souches. L'importation de toute espèce est conditionnée par les résultats d'une enquête préliminaire dont l'objet est de rechercher les exigences écologiques de l'espèce et la situation épidémiologique dans son milieu d'origine. Le travail du LGP et de ses partenaires a été partagé en différentes actions: 1. Recherche de contacts dans divers pays étrangers, en vue de l'importation d'huîtres présentant des caractéristiques intéressantes. 2. Importation : L'importation se fait selon les normes du CIEM (Conseil International pour l'Exploration de la Mer) : contrôles pathologiques...
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Marqueurs moléculaire; Marqueurs populations; Crassostrea.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15520/12907.pdf
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Bonamia-like parasite found in the Suminoe oyster Crassostea rivularis reared in France ArchiMer
Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,...
Tipo: Text Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf
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Bonamia-like parasite found in the Suminoe oyster Crassostrea rivularis reared in France ArchiMer
Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production. Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-Like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea....
Tipo: Text Palavras-chave: Crassostrea rivularis; Suminoe oyster; Bonamiosis; Bonamia-like parasite.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00446/55795/62652.pdf
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Point sur le programme huître plate - Complément au compte-rendu de réunion REGEMO sur l'Amélioration Génétique de l'huître plate du 11 mars 1998 ArchiMer
Goyard, Emmanuel; Lapegue, Sylvie; Gerard, Andre; Boudry, Pierre.
Ce document vient compéter le rapport REGEMO sur l'huître plate. Une première version visait essentiellement à faciliter le transfert de responsabilité du programme en cours. Celle-ci, épurée des aspects pratiques purement internes, se propose de faire le point sur les familles produites en 1998, et la continuité du programme d'amélioration.
Tipo: Text Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00049/15980/13422.pdf
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